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The growth of solid tumors and their metastases is dependent on angiogenesis, the formation of new capillaries from pre-existing vessels. Angiogenesis is regulated by a shift in the balance of endogen angiogenic (bFGF, aFGF, VEGF vs.) and antiangiogenic factors (endostatin, angiostatin, interferon vs.) in human body. Endostatin, one of the most potent negative regulators of angiogenesis, is naturally occurring inhibitor of angiogenesis capable of inhibiting tumor growth and their metastases. It is reported that Endostatin used between 10-20 days 0.3-20 mg/kg/day doses giving intraperitoneal or subcutan has

coused antiangiogenic effect in the experimental cancers carried out on the lab animals. In our study, DMH injected 20mg/kg/week dose by subcutan induces colon cancer on 30 Balb-c male mice durig the 12 weeks. 12 weeks after the last DMH dose, 7 ųg/day endostatin was injected subcutaneously to seven animals to search its antitumor effect. As a result of our study, it was found out that tumor occured 100% in the group of DMH-treated mice and endostatin-treated mice. While there are 77 lesions in the group of DMH, there are 57 lesions in the group of endostatin. It was found out that there is 36,3% low displasi, 22% high displasi and 41,5% carcinom of the 77 lesions in the group of DMH. On the other hand there is 31,5% low displasi, 17,5% high displasi and 50,8% carcinom in the group of endostatin. When we have a look the distribution of the lesions in colon, 74% of the lesions of DMH group and 75,4% of the lesions of endostation group occured in the distal colon. In the end of our study, we noticed that the number of lesions decreaed in the group of endostatin, considering the number of the lesions in the group of DMH. But there was no statistically difference between the mice treated with endostatin and those treated with DMH.

Ömür Karaca
Farelerde deneysel olarak oluşturulan kolon kanseri üzerine endostatin’in etkileri · 2008 · 87 sayfa.
Danışman: Doç. Dr. Harun Ülger

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